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- Beginners Guide to Mass Spectral Interpretation (Hardcover).
- A beginners guide to mass spectral interpretation.
Pages and cover are clean and intact. Used items may not include supplementary materials such as CDs or access codes. May show signs of minor shelf wear and contain limited notes and highlighting. Seller Inventory Terrence A. Publisher: Wiley , This specific ISBN edition is currently not available. View all copies of this ISBN edition:. Synopsis About this title This book is a logical, step-by-step guide to identification of organic compounds by mass spectrometry.
From the Back Cover : This book, the first to be dedicated solely to interpretation of electron impact mass spectra, is a logical, step-by-step guide to identification of organic compounds by mass spectrometry. About the Author : General Readers. Students in nutrition and allied health "About this title" may belong to another edition of this title. Shows some Learn more about this copy. Other Popular Editions of the Same Title.
A Beginner’s Guide: How to Interpret Gas Chromatography Mass Spectrometry Results
Search for all books with this author and title. Customers who bought this item also bought. Stock Image. MS works by ionizing, or bestowing a net charge, on a sample of molecules and then sorting the ions based on their mass-to-charge ratio. The charged molecules are then guided by electromagnetic attraction or repulsion to a detector mechanism. Aspiring chemists and biologists, as well as anyone interested in gaining a greater understanding of these fields, can benefit from a greater understanding of MS. This Instructable will provide a set of instructions for the reading and comprehension of a simple mass spectrum of a halogenated alkane haloalkane , or a compound containing only hydrogen, carbon and a halogen.
Haloalkanes will be analyzed both because of their wide use as chemical solvents and because understanding of their spectra forms a good baseline for future learning in the MS field. The above information will be explained in more detail in the coming steps to share insight into how a mass spectrometrist can use a mass spectrum to determine the identity of an unknown chemical sample. Experience Required : High school level knowledge of chemistry i.
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Mass Spectrometry Digital Resource Center
Add a Teacher Note to share how you incorporated it into your lesson. The molecular ion represents the entire molecule in question, prior to any fragmentation. Ionization, specifically electron impact EI ionization, is used to remove an electron from an analyte molecule so that it can be analyzed by the electrical and magnetic fields of the mass spectrometer. It is therefore important to first identify the molecular complete ion.
Find and record this value in your notebook. Fortunately, individual molecules have relatively unique EI fragmentation patterns. The different peaks on a mass spectrum reveal the compounds identity, so, as shown below, a mass spectrometrist should identify all major spectral peaks.
How to Read a Simple Mass Spectrum
A major peak is the most abundant peak within a cluster of smaller peaks. For this introductory Instructable, the largest most abundant peak in each cluster will represent the entire cluster. The most abundant peak is, by naming convention, the base peak.
Smaller peaks clustered around each major peak are largely present due to differences in which of the two fragments retains the ion, gain or loss of protons, and naturally occurring elemental isotopes. These mass differences represent the gain or loss of a methyl -CH3 or methylene -CH2- from the larger ion.
Write these values down in your notebook.
- A Beginner's Guide to Mass Spectral Interpretation by Terrence A. Lee
Mass differences between major peaks will often exceed the 14 or 15 mass units representing a methyl or methylene carbon. Common halogens Group 17 on the Periodic Table include fluorine, chlorine, bromine and iodine. These can essentially replace one hydrogen-to-carbon bond of an alkane and dramatically alter its chemical and physical properties. Luckily, singularly halogenated alkanes have easily identifiable mass spectra see iodobutane spectra below.
Check your list of mass differences created in Step 3. Write this halogen down in your notebook.
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